Epi Multiome
Epi Multiome (10x Chromium) simultaneously measures 3' gene expression and open chromatin (ATAC) from the same single nucleus, linking each cell's transcription to its regulatory landscape.
Specification
| Vendor | 10x Genomics |
|---|---|
| Modality | single-nucleus multiome: 3' gene expression + chromatin accessibility (ATAC), same nucleus |
| Tissue | nuclei suspensions (fresh, frozen or cryopreserved cells/tissue) |
| Input | Fresh or frozen tissue, cells, or nuclei; the Multiome assay cannot take FFPE |
| Multiplexing | No officially supported multiplexing (single sample per reaction) |
| Species | species-agnostic (human, mouse and others) |
| Billing | By consultation (free) |
Accreditations & experience
The Spatial Technologies Unit is an accredited service provider and among the first facilities worldwide certified through 10x Genomics' Core Lab Training program. See the Accreditations page.
What it measures
- 3' gene expression and chromatin accessibility (ATAC) from the same nucleus.
- Two libraries per sample (gene expression and ATAC), analysed together in Cell Ranger ARC.
Input and workflow
- Input: nuclei suspensions only, from fresh, frozen or cryopreserved cells and tissue.
- Transposition tags open chromatin; nuclei are then partitioned into droplets on the Chromium X/iX.
- Gene expression and ATAC libraries are sequenced separately on Illumina instruments.
What you receive
- FASTQ files (gene expression and ATAC)
- Cell Ranger ARC outputs: gene-expression and ATAC feature-barcode matrices
- ATAC fragments file and peak matrix
- web-summary QC report; MD5 checksums at transfer
Access and priority
Internal clients (BIDMC, DF/HCC), external academic and non-profit clients, and industry clients all have equal priority. Projects begin upon sample receipt.