Epi ATAC
Epi ATAC (10x Chromium) profiles genome-wide chromatin accessibility in single cells, mapping open regulatory regions and transcription-factor motif accessibility.
Specification
| Vendor | 10x Genomics |
|---|---|
| Modality | single-cell ATAC: genome-wide open chromatin accessibility |
| Tissue | nuclei suspensions (fresh, frozen or cryopreserved cells/tissue) |
| Input | Nuclei from fresh or frozen tissue; Epi ATAC cannot take FFPE |
| Cells per sample | 500-10,000 cells/sample |
| Multiplexing | Singleplex, or up to 96-well multiplexing for high throughput |
| Species | species-agnostic |
| Billing | By consultation (free) |
Accreditations & experience
The Spatial Technologies Unit is an accredited service provider and among the first facilities worldwide certified through 10x Genomics' Core Lab Training program. See the Accreditations page.
What it measures
- Genome-wide open chromatin accessibility at single-cell resolution.
- Supports analysis of transcription-factor motif accessibility and gene-regulatory inference.
Input and workflow
- Input: nuclei suspensions only, from fresh, frozen or cryopreserved cells and tissue.
- A transposase (Tn5) inserts adapters into open chromatin; nuclei are partitioned on the Chromium X.
- A single ATAC library per sample is sequenced on Illumina instruments.
What you receive
- FASTQ files
- Cell Ranger ATAC outputs: fragments file and peak-barcode matrix
- web-summary QC report; MD5 checksums at transfer
Access and priority
Internal clients (BIDMC, DF/HCC), external academic and non-profit clients, and industry clients all have equal priority. Projects begin upon sample receipt.